Description
BSC:Bio-Safety cabinet
avoid damage by harmful substances, protective role.
hazardous industries ideal for devices.
requirements.
Features
Selection of a biological safety cabinet (BSC)
BSC category |
Laboratory type |
Type of protection |
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Class I |
Groups 1–3, microorganisms in Risk; |
Operator, environment |
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Class Ⅱ A1 |
Groups 1–3, microorganisms in Risk |
Operator, environment, product |
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Class Ⅱ A2 |
Groups 1–3, microorganisms in Risk |
Operator, environment, product |
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Class Ⅱ B1 |
Groups 1–3, microorganisms in Risk; |
Operator, environment, product |
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Class Ⅱ B2 |
Groups 1–3, microorganisms in Risk; |
Operator, environment, product |
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Class III |
Groups 1–3, microorganisms in Risk; |
Operator, environment, product |
The categories of Bio Safety Cabinet are defined and classified according to the National Sanitation Foundation Standard, NSF/ANSI 49.
◎ The Class I BSC is designed primarily for the protection of operating personnel
BSC grades |
Inlet air velocity |
% of air flow |
Exhaust system |
Applications |
||
m/s |
internal |
Exhausted |
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|
0.38 |
0 |
100 |
External |
It is used for laboratories which are work with nonvolatile, radionuclide/chemical protection, microorganisms in Risk. |
◎ The Class II BSC is designed not only to provide personnel protection but also to protect work surface materials from being contaminated by the room air which is drawn into the cabinet. And it is used for protecting the laboratory environment as well. It is acceptable for working with medium level risk tests. And according to the National Sanitation Foundation Standard, NSF/ANSI 49(2002), it can be defined and classified as 4 types. Their features are compared as below.
BSC grades |
Inlet air velocity |
% of air flow |
Exhaust system |
Applications |
||
m/s |
internal |
Exhausted |
||||
|
0.38 |
70 |
30 |
Internal or external |
It is used for laboratories which are work with nonvolatile, radionuclide/chemical protection, microorganisms in Risk. |
|
|
0.5 |
70 |
30 |
|||
|
0.5 |
30 |
70 |
External |
It is used for laboratories which are work with lightly volatile, radionuclide/chemical protection, microorganisms in Risk. |
|
|
0.5 |
0 |
100 |
◎ Class III biological safety cabinets are suitable for work in Bio-safety Level 3 and 4 laboratories and provide the protection for the highest hazardous level of the Risk Group 3, Risk Group 4, or above. It virtually is a sealed gas tight cabinet. Moreover, its supply air is HEPA- filtered and exhaust air passes through two HEPA filters which keep the cabinet interior under negative pressure consequently. The only way of accessing to the work surface for operation is by means of heavy duty rubber gloves, which are attached to ports in the cabinet virtually.
BSC grade |
Inlet air velocity |
% of air flow |
Exhaust system |
Applications |
||
m/s |
internal |
Exhausted |
||||
Class III |
N/A |
0 |
100 |
Exhaust air should pass through two filters. |
It is used for laboratories which are work with lightly volatile, toxic, radionuclide protection, microorganisms in Risk. |